Table of Contents

HK J Paediatr (New Series)
Vol 2. No. 2, 1997

HK J Paediatr (New Series) 1997;2:177

Proceedings of Scientific Meeting

Rat Duodenum Does Not Go Through "Solid Core" Stage in Its Embryological Development

W Cheng, PKH Tam

HK J Paediatr (new series) 1997;2:175-186

Hong Kong Paediatric Society 35th Anniversary Scientific Meeting September 6,1997

Purpose : Embryological development of duodenum is believed to go through a "solid core stage which subsequently vacuolizes. The failure of vacuolization is thought to be the cause of duodenal atresia. This study examines the embryological development of rat duodenum.

Methods : Twelve time mated Sprague-Dawley rats were sacrificed from day 8 to day 20 of gestation, one for each gestational day. The rat fetuses were harvested. The stomach, duodenum and proximal jejunum of the fetuses of day 15 to day 20 were dissected out under microscope. Of the fetuses of day 11 to day 14 of gestation, the whole bodies were removed. For gestation day 8 to day 10, the embryos as well as the placentas were removed. These specimens were fixed with formalin, sectioned, stained with haematoxylin and eosin and examined under microscope. The dimensions of three sets of duodenal diameters and the lumen diameters of each gestational dates were measured.

Results : The foregut started to form at the gestation stage of day 10. The lumen was narrowest at day 12 and 13, but the duodenum did not become "solid core" (Table). No vacuolization was detected through out the development of the duodenum.

Gestational Date 11 12 13 14 15 16 17 18 19 20
Mean lumen diameter (mm) 0.07 0.01 0.01 0.07 0.13 0.30 0.33 0.30 0.37 0.40
Mean outer diameter (mm) 0.10 0.20 0.27 0.30 0.33 0.53 0.57 0.58 0.77 0.80
Lumen to outer diameter ratio 0.67 0.03 0.05 0.22 0.40 0.56 0.59 0.51 0.48 0.50
Table The mean duodenal lumen diameters, the mean duodenal outer diameters and the ratio between the two.

Conclusions : Embryological development of rat duodenum does not go through a "solid core" stage. This finding challenges the traditional explanation for the pathogenesis of duodenal atresia and suggests the need of further studies for clarification.


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