Table of Contents

HK J Paediatr (New Series)
Vol 5. No. 2, 2000

HK J Paediatr (New Series) 2000;5:139-142

Original Article

Resuscitation of Asphyxiated Fetal Rats with Room Air or Oxygen: Changes of Cerebral Intraand Extra-cellular Calcium
宮內窘迫胎鼠空氣復甦與高氧復甦的比較

SH Nong, YM Xie, XS Huang


Abstract

Objective: To compare the effects of resuscitation using room air or oxygen on hypoxic damage of fetal rat brains. Methods: Thirty-five fetal rats of 20-day gestational age were randomly divided into three groups: sham operation (control, n=11), room-air resuscitation (n=10), and oxygen (concentration 92.8%) resuscitation (n=14). Fetal rats in the latter two groups suffered from ischemia and hypoxia inutero resulting from interruption of placental circulation. After recirculation, intra- and extra-cellular concentrations of calcium, sodium, and potassium in the brains were measured in each group. Results: Intracellular free calcium concentration of fetal rat brains was similar between the room-air resuscitation group (552.08±93.50 nmol/L) and the oxygen resuscitation group (520.61±79.08 nmol/L), and both were significantly higher than that in the control (315.27±86.88 nmol/L) (P<0.001). There was no difference in the total concentrations of calcium, sodium, or potassium among the three groups. Conclusion: Resuscitation with room air or 92.8% oxygen had a similar effect on the parameters measured, suggesting that resuscitation of asphyxiated neonates using room air might not be inferior to that using high-concentration oxygen.

目的:比較窒息時的空氣與純氧復甦的效果,為窒息復甦方案的選擇提供依據。方法:將 35 隻胎齡為 20 天的 SD 大鼠胎鼠隨機分為 3 組,即假手術組(對照組,C 組)11 隻,製成宮內窘迫模型而給予空氣復甦者作為空氣復甦組(A 組)10 隻,給予 92.8% 濃度氧復甦者為高氧復甦組(Ox 組)14 隻。監測各組胎鼠腦細胞內外鈣、鈉、鉀含量的變化。結果:空氣復甦組與高氧復甦組的腦細胞內游離鈣離子濃度(分別為 552.08±93.50 和 520.61±79.08 nmol/L)基本一樣,均明顯高於對照組(315.27± 86.88 nmol/L)(P<0.001)。各組腦組織總鈣、鈉、鉀含量無顯著性差異(P<0.05)。結論:應用空氣或 92.8% 濃度氧進行窒息復甦時,復甦效果相似,提示臨床上可考慮應用空氣代替純氧對窒息新生兒進行復甦。

Keyword : Fetal hypoxia; Oxygen; Resuscitation; Room air

 
 

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