YY Luo, S Chen, JD Yu, XL Shu, Q Cao, J Chen

Abstract

Objectives: To observe single nucleotide polymorphisms (SNPs) of vitamin D metabolism-related factors, vitamin D receptor (VDR) and vitamin D binding protein (DBP), in Crohn's disease (CD). Methods: 121 patients with CD and 381 controls were enrolled. SNP (rs731236) in VDR gene and SNPs (rs4588, rs7401 and rs2282679) in DBP gene were typed in patients with CD and controls by gene sequencing. Results: In our case-control cohort, no significant difference was observed on CD risk for any of the four SNPs (rs731236, rs4588, rs7401 and rs2282679) in vitamin D metabolism-related genes (P>0.05). Furthermore, there was no association between CD susceptibility and the haplotypes of DBP gene (P>0.05). Conclusions: Our study suggests that the four SNPs (rs731236, rs4588, rs7401 and rs2282679) in vitamin D metabolism-related genes may have no correlation with susceptibility of CD in Chinese Han population. SNP (rs731236) in VDR gene may play a role in the aetiology of CD among affected males. However, our findings need to be confirmed in multi-centre studies.

目的：觀察CD中維生素D代謝相關因數、維生素D受體(VDR)、維生素D相關蛋白(DBP)的單核苷酸多態性(SNPs)。方法：實驗組121名CD患者，對照組381名，用基因測序的方法對各組VDR基因的SNP(rs731236)、DBP基因的SNPs(rs731236、rs4588、rs7401和rs2282679)進行分型。結果：在我們的病例對照研究中，維生素D代謝相關的四種SNPs(rs731236、rs4588、rs7401和rs2282679)兩組沒有差異(P>0.05)，而且CD易感性與DBP基因的單倍性沒有關聯(P>0.05)。結論：研究表明，中國漢族人維生素D代謝相關的四種SNPs(rs731236、rs4588、rs7401和rs2282679)與CD易感性無相關，VDR基因的SNP(rs731236)在對受影響男性的CD的病因中發揮作用。但是我們的發現需要在多中心研究中證實。